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Updated: Aug 29, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
A myosin inhibitor impairs auxin-induced cell division
Carola Holweg1, Anne Honsel, Peter Nick
1Institut für Biologie II, Albert-Ludwigs-Universität Freiburg, Schänzlestrasse 1, 79104 Freiburg, Federal Republic of Germany.
Abstract:
The role of myosins for auxin-induced cell division was probed using the inhibitor 2,3-butanedione monoxime in the tobacco cell line VBI-0, where cell elongation and division are axially aligned under the control of auxin. A morphometric analysis revealed that cell division is blocked in a dose-dependent manner, whereas cell expansion continued. In addition, the polarity of terminal cells was impaired resulting in malformed, pear-shaped cells. Early effects of the inhibitor are aberrant features of the cytoarchitecture including a block of vesicle transport, a diffuse broadening of cross walls, and the disorganization of the actin cytoskeleton. The findings are discussed with respect to a possible role of myosins as link between vesicle flow and signal control of cell division.
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