[Conjugation mapping of Pseudomonas mendocina bacteria]
S L Vasilenko1, N P Maksimova, M A Titok
1Department of Genetics, Belarus State University, Minsk, 220050 Belarus. vasylenko@bsu.by
Genetika
|January 13, 2004
Abstract:
Donor strains of the Hfr type were isolated using plasmid pRK2013 with transposons Tn10 and Tn5 as a chromosome-mobilizing factor. The isolated strains were shown to promote transfer of donor chromosome from different origins in different directions during isogenic matings of Pseudomonas mendocina bacteria. The created collection of donors and polyauxotrophic recipient bacteria permitted mapping 26 genetic determinants on the bacterial chromosome and identifying the genome of these microorganisms as a circular DNA molecule.
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