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Updated: Aug 8, 2026

A Direct, Early Stage Guanidinylation Protocol for the Synthesis of Complex Aminoguanidine-containing Natural Products
Published on: September 9, 2016
[Guanidine hydrochloride mediated unfolding of a carbonic anhydrase molten globule]
V P Kutyshenko1, D A Prokhorov
1Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, Moscow Oblast, 142290 Russia. kutyshenko@rambler.ru
Abstract:
Guanidine hydrochloride-induced unfolding of a carbonic anhydrase molten globule was studied by high-resolution NMR spectroscopy. The study resulted in estimation of the number of water and denaturant molecules bound to the molten globule at various denaturant concentrations in solution. When compared with the data on unfolding of native carbonic anhydrase, these estimates indicate that the unfolding is underlain by an increased local concentration of the denaturant near the protein molecule, which results from the increased ratio between guanidine hydrochloride-bound and protein-bound waters.
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