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Long-term kinetic vitreous fluorophotometry.

L L Knudsen¹, T Olsen, F Nielsen-Kudsk

¹Department of Ophthalmology, University of Arhus, Denmark.

Acta Ophthalmologica

|October 1, 1992

Summary

This study used fluorophotometry to analyze sodium fluorescein kinetics in plasma and vitreous humor. Results reveal slow fluorescein elimination from the eye, suggesting active transport across the blood-ocular barrier.

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Area of Science:

Ophthalmology
Pharmacokinetics
Biomedical Engineering

Background:

The blood-ocular barrier (BOB) tightly regulates the passage of substances into the eye.
Understanding fluorescein transport dynamics is crucial for diagnosing and monitoring ocular conditions.

Purpose of the Study:

To quantify the kinetic parameters of sodium fluorescein distribution and elimination in human plasma and vitreous humor.
To investigate the transport mechanisms across the blood-ocular barrier using kinetic analysis.

Main Methods:

Intravenous injection of sodium fluorescein in 14 normal subjects.
Fluorophotometric measurements of plasma and vitreous fluorescence up to 24 hours.
Kinetic two-compartmental analysis to determine transfer rate constants (K12, K21, Kin, Kout).

Main Results:

Plasma fluorescein exhibited a half-life of 3.01 hours.
Vitreous fluorescence peaked at 2-5 hours and declined slowly (t1/2 = 9.6 hours).
Permeation into the eye (Kin = 0.66 h-1) was significantly higher than plasma-to-peripheral tissue transfer (K12), and vitreous elimination (Kout = 0.072 h-1) was significantly lower than peripheral tissue-to-plasma return (K21).

Conclusions:

Sodium fluorescein demonstrates poor penetration into the vitreous (permeability index 3.5%).
Higher Kin than K12 suggests active transport at the blood-ocular barrier.
Slower Kout than K21 indicates prolonged vitreous retention.
Kinetic fluorophotometry is a valuable tool for studying blood-ocular barrier function.

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