Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone morphogenetic protein induces bone in the squirrel monkey, but bone matrix does not.

P Aspenberg1, E Wang, K G Thorngren

  • 1Department of Orthopedics, Lund University Hospital, Sweden.

Acta Orthopaedica Scandinavica
|December 1, 1992
PubMed
Summary

Adding bone morphogenetic protein-2 (BMP-2) to demineralized bone matrix (DBM) successfully induced bone formation in most monkeys. This suggests higher concentrations of inductive proteins may overcome challenges in higher animals.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Intrathecal herpes simplex virus type 1 amplicon vector-mediated human proenkephalin reduces chronic constriction injury-induced neuropathic pain in rats.

Molecular medicine reports·2011
Same author

[Effect of intrathecal ketamine injection on protein kinase C expression in the spinal dorsal horn of rats with formalin-induced pain].

Nan fang yi ke da xue xue bao = Journal of Southern Medical University·2011
Same author

Concurrent strength and endurance training improves physical capacity in patients with peripheral arterial disease.

Scandinavian journal of medicine & science in sports·2011
Same author

Magnetic stochasticity in gyrokinetic simulations of plasma microturbulence.

Physical review letters·2011
Same author

Toll-like receptor 4 signaling confers cardiac protection against ischemic injury via inducible nitric oxide synthase- and soluble guanylate cyclase-dependent mechanisms.

Anesthesiology·2011
Same author

Factors associated with sustained virological response in liver transplant recipients with recurrent hepatitis C.

Transplantation proceedings·2010

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Orthopedic Research

Background:

  • Demineralized bone matrix (DBM) is known to induce bone formation in rodents.
  • However, its efficacy in larger animals like dogs and primates has been inconsistent or negative.
  • Previous studies showed DBM failed to induce bone in squirrel monkeys, despite success in nude rats.

Purpose of the Study:

  • To investigate if augmenting demineralized bone matrix (DBM) with recombinant human bone morphogenetic protein-2 (BMP-2) can enhance bone induction in primates.
  • To compare the efficacy of augmented DBM in squirrel monkeys and nude rats.

Main Methods:

  • Demineralized bone matrix (DBM) was combined with recombinant human bone morphogenetic protein-2 (BMP-2).
  • Implants were tested in squirrel monkeys and nude rats.

Related Experiment Videos

  • Bone formation was assessed using histology and calcium content analysis.

    • Main Results:

    • Bone formation was observed in 10 out of 12 monkeys treated with DBM + BMP-2, confirmed by histology and calcium content.
    • In 4 monkeys, the induced bone volume was less than the original implant size.
    • DBM alone induced minimal bone in 2 out of 10 monkeys.
    • All DBM controls and augmented implants successfully induced bone in nude rats.

    Conclusions:

    • Augmenting demineralized bone matrix (DBM) with recombinant human bone morphogenetic protein-2 (BMP-2) can induce bone formation in squirrel monkeys.
    • Higher concentrations of inductive proteins, like BMP-2, may be necessary to overcome limitations of DBM in inducing bone in higher animals.
    • This approach shows potential for improving bone regeneration strategies in primate models.