Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changes in hippocampal SNAP-25 expression following afferent lesions.

María J Ramírez1, William G Honer, Stephen L Minger

  • 1Department of Pharmacology, School of Medicine-University of Navarra, Irunlarrea 1, 31008 Pamplona, Spain. mariaja@unav.es

Brain Research
|January 13, 2004
PubMed
Summary

Synaptosomal-associated protein 25 (SNAP-25) levels initially decrease after hippocampal afferent lesions but normalize within a month, suggesting SNAP-25 changes may mark early synaptic loss.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Comparison Between Lifestyle Interventions and Usual Care Among Indian Adults With Hypertension: A Systematic Review and Meta-Analysis.

Cureus·2026
Same author

Burnout, associated factors and coping strategies among nursing professionals in Kerala, India: a cross-sectional study.

BMJ open·2026
Same author

From neglect to empowerment: impact of vocational rehabilitation on quality of life among disabled filarial lymphoedema patients in a brugian-endemic region of South India-a mixed methods evaluation.

Transactions of the Royal Society of Tropical Medicine and Hygiene·2026
Same author

Astrocytes as Metabolic Sensors Orchestrating Energy-Driven Brain Vulnerability in Alzheimer's Disease.

Journal of neurochemistry·2025
Same author

Biochemical and Structural Response to Lenvatinib in Metastatic Parathyroid Carcinoma: Case Report.

Clinical medicine insights. Endocrinology and diabetes·2025
Same author

Prevalence and Risk Factors of Hypertension Among Adults in Ernakulam District, Kerala: A Cross-Sectional Study.

Cureus·2025

Area of Science:

  • Neuroscience
  • Cell Biology
  • Neurochemistry

Background:

  • Synaptic plasticity and integrity are crucial for hippocampal function.
  • Synaptosomal-associated protein 25 (SNAP-25) is a key component of the presynaptic terminal.
  • Understanding molecular changes following neuronal input loss is vital for neurodegenerative disease research.

Purpose of the Study:

  • To investigate the dynamic changes in SNAP-25 expression in the rat hippocampus following afferent pathway lesions.
  • To determine if cholinergic and glutamatergic inputs differentially affect SNAP-25 levels.
  • To assess the role of SNAP-25 as a potential biomarker for synaptic loss.

Main Methods:

  • Rats underwent surgical lesions to remove specific afferent inputs to the hippocampus (entorhinal cortex, septohippocampal pathway).

Related Experiment Videos

  • Immunohistochemistry was used to quantify SNAP-25 levels in hippocampal tissue at various time points post-lesion.
  • Comparative analysis of SNAP-25 normalization following single and superimposed lesions was performed.

    • Main Results:

    • A significant reduction in SNAP-25 immunohistochemistry was observed immediately after combined glutamatergic and cholinergic afferent deafferentation.
    • SNAP-25 levels returned to baseline within one month following the initial afferent lesions.
    • Subsequent cholinergic lesions superimposed on long-term entorhinal cortex lesions did not impede the normalization of SNAP-25 levels.

    Conclusions:

    • Transient reductions in SNAP-25 levels following afferent deafferentation are an early indicator of synaptic changes in the hippocampus.
    • The normalization of SNAP-25 suggests adaptive mechanisms or compensatory processes occur post-lesion.
    • SNAP-25 dynamics may serve as a sensitive marker for assessing synaptic integrity and recovery in the hippocampus.