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Cloning and sequence analysis of the Candida utilis HIS3 gene
Liliana Basabe1, Iveldris Domínguez, Francisco P Chávez
1Center for Genetic Engineering and Biotechnology, Agriculture Division, 31 Ave /158 & 190, PO Box 6162, Havana 10600, Cuba. liliana.basabe@cigb.edu.cu
Biomolecular Engineering
|January 13, 2004
Abstract:
A DNA fragment, carrying the Candida utilis HIS3 gene, has been isolated from a genomic DNA library by complementation of the E. coli hisB mutant. Its nucleotide sequence was determined and it predicts a single open reading frame of 675 bp (224 aa). The deduced amino acid sequence is highly homologous to other yeast and fungi HIS3 genes.