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Automated procedure for improving the RNA isolation step in viral load testing for human immunodeficiency virus
Min Xu1, Yung Chan, Steven H Fischer
1Department of Laboratory Medicine, Warren Grant Magnuson Clinical Center, National Institutes of Health, Bethesda, Maryland 20892-1508, USA.
Journal of Clinical Microbiology
|January 13, 2004
Abstract:
An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation procedure.