Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Microbial P450 enzymes in biotechnology.

V B Urlacher1, S Lutz-Wahl, R D Schmid

  • 1Institut für Technische Biochemie, Universität Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.

Applied Microbiology and Biotechnology
|January 13, 2004
PubMed
Summary

Cytochrome P450 monooxygenases offer selective oxidation but face challenges. Recent advances in electron supply and protein engineering show promise for their industrial biocatalytic applications.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular LEGO by domain-imprinting of cytochrome P450 BM3.

Colloids and surfaces. B, Biointerfaces·2018
Same author

Preservation of immobilized bacterial cell-matrix by drying for direct use in microbial sensors.

World journal of microbiology & biotechnology·2014
Same author

Recombinant production of hyperthermostable CelB from Pyrococcus furiosus in Lactobacillus sp.

Applied microbiology and biotechnology·2012
Same author

Recombinant Production of Horseradish Peroxidase Conjugates with Fab Antibodies in Pichia pastoris for Analytical Applications.

Acta naturae·2012
Same author

Multiparametric determination of genes and their point mutations for identification of beta-lactamases.

Biochemistry. Biokhimiia·2011
Same author

Global transcription and metabolic flux analysis of Escherichia coli in glucose-limited fed-batch cultivations.

Applied and environmental microbiology·2008

Area of Science:

  • Biocatalysis
  • Enzyme Engineering
  • Organic Chemistry

Background:

  • Biooxidations offer selective oxygen introduction difficult in synthetic chemistry.
  • Cytochrome P450 monooxygenases (P450s) are a large enzyme superfamily with diverse reactions.
  • Current P450 applications are limited by complexity, low activity, stability, and cofactor requirements.

Purpose of the Study:

  • To review the potential of bacterial P450s in biocatalysis.
  • To highlight recent progress in overcoming P450 limitations for industrial use.

Main Methods:

  • Investigating cost-efficient electron supply methods (mediators, electrodes, enzymatic).
  • Applying protein design and directed evolution to enhance P450 activity and selectivity.

Main Results:

  • Bacterial P450s show promise as biocatalysts.
  • Methods for continuous electron supply and enzyme improvement are advancing.

Conclusions:

  • Overcoming P450 limitations is crucial for their broader industrial application.
  • Continued research in enzyme engineering and cofactor regeneration will enhance biocatalytic processes.

Related Experiment Videos