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Updated: Aug 29, 2026
Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes
Published on: July 30, 2020
Preparation and property analysis of a hepatocyte targeting pH-sensitive liposome
Si-Yuan Wen1, Xiao-Hong Wang, Li Lin
1Beijing Institute of Radiation Medicine, Beijing 100850, China.
Aim:
To develop a hepatocyte targeting pH-sensitive liposome for drug delivery based on active targeting technology mediated by asialoglycoprotein receptors.
Methods:
Four types of targeting molecules with galactose residue were synthesized and mixed with pH-sensitive lipids DC-chol/DOPE to prepare liposome with integrated property of hepatocyte specificity and pH sensitivity. Liposome 18-gal was selected with the best transfection activity through cellular uptake experiment. Property analysis was made through experiments of competitive inhibition of receptors, red blood cell hemolysis, in vitro cytotoxicity test by MTS assay and mediation of inhibitory effects of antisense phosphorothioate ODN on gene expression, etc.
Results:
Liposome 18-gal had the desired properties of hepatocyte specificity, pH sensitivity, low cytotoxicity, and high transfection efficiency.
Conclusion:
Liposome 18-gal can be further developed as a potential hepatocyte- targeting delivery system.
