Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fibronectin matrix assembly regulates alpha5beta1-mediated cell cohesion.

Elizabeth E Robinson1, Ramsey A Foty, Siobhan A Corbett

  • 1Department of Surgery, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, New Jersey 08903, USA.

Molecular Biology of the Cell
|January 14, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-cell mechanical analysis and tension quantification via electrodeformation relaxation.

Physical review. E·2021
Same author

Rutgers Robert Wood Johnson Medical School.

Academic medicine : journal of the Association of American Medical Colleges·2021
Same author

Coherent Timescales and Mechanical Structure of Multicellular Aggregates.

Biophysical journal·2018
Same author

Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo.

International journal of molecular sciences·2018
Same author

Dexamethasone-mediated inhibition of Glioblastoma neurosphere dispersal in an ex vivo organotypic neural assay.

PloS one·2017
Same author

Inhibition of glioblastoma dispersal by the MEK inhibitor PD0325901.

BMC cancer·2017

The extracellular matrix (ECM) plays a key role in three-dimensional (3D) cell aggregation. Fibronectin (FN) matrix assembly promotes integrin-mediated tissue cohesion and regulates tissue architecture.

Area of Science:

  • Cell Biology
  • Biomaterials Science
  • Tissue Engineering

Background:

  • Integrin-extracellular matrix (ECM) interactions are well-studied in 2D cultures.
  • The role of ECM in promoting intercellular cohesion in 3D environments is less understood.
  • Previous work demonstrated alpha5beta1-integrin mediates intercellular cohesion in 3D cellular aggregates.

Purpose of the Study:

  • To investigate the mechanism of alpha5beta1-integrin-mediated cohesivity in 3D cellular aggregates.
  • To determine the role of fibronectin (FN) matrix assembly in alpha5beta1-integrin-mediated cell aggregation, compaction, and cohesion.

Main Methods:

  • Utilized chimeric alpha5beta1-integrin-expressing cells cultured as multilayer aggregates.
  • Modified the alpha5 subunit cytoplasmic domain to investigate its role in integrin function.

Related Experiment Videos

  • Assessed cell aggregation, compaction, and cohesion, correlating these with FN matrix assembly.

    • Main Results:

    • Cells can assemble an FN matrix that promotes integrin-mediated aggregate compaction and cohesion without external mechanical support.
    • Inhibition of FN matrix assembly disrupts intercellular associations, leading to cell dispersal.
    • The cytoplasmic domain of the alpha5 subunit is crucial for alpha5beta1-integrin-mediated cell cohesion.

    Conclusions:

    • FN matrix assembly significantly contributes to tissue cohesion.
    • This process represents an alternative mechanism for regulating tissue architecture.
    • Alpha5beta1-integrin plays a critical role in mediating these FN matrix-dependent cohesive processes.