Related Experiment Video
Updated: Aug 29, 2026
A 3D Spheroid Model for Glioblastoma
Published on: April 9, 2020
Effect of single dose irradiation on human glioblastoma spheroids in vitro
Fabian Fehlauer1, Lukas J A Stalpers, John Panayiotides
1Department of Radiation Oncology, University of Hamburg, Martinistrasse 52, 20246 Hamburg, Germany. fehlauer@uke.uni-hamburg.de
Abstract:
Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-beta and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-beta and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.
