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Electrodes modified with monoolein cubic phases hosting laccases for the catalytic reduction of dioxygen
Paweł Rowiński1, Renata Bilewicz, Marie-José Stébé
1Department of Chemistry, University of Warsaw, Pasteura 1, 02-093 Warsaw, Poland, and Equipe Physico-chimie des Colloïdes, UMR 7565 CNRS, Faculté des Sciences, Université Henri Poincaré Nancy 1, BP 239, 54506 Vandoeuvre-lès-Nancy, Cedex, France.
Abstract:
An enzyme-catalyzed process has been used for dioxygen monitoring. The enzymes were two different laccases (p-diphenol:dioxygen oxidoreductases), chosen as catalysts for dioxygen reduction. The laccases were immobilized in a liquid crystalline cubic phase formed with monoolein. The structures of the cubic phases, both with and without enzymes, were established using small-angle X-ray scattering. The catalytic reduction of dioxygen was performed using a glassy carbon electrode modified with cubic phases containing the enzymes. The modified electrode was used as a dioxygen sensing system, based on the increasing reduction current of a suitable electrochemical probe in the presence of dioxygen.
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