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Affinity two-phase partitioning in acoustically levitated drops.

Sabina Santesson1, Irene Barinaga-Rementeria Ramírez, Peter Viberg

  • 1Technical Analytical Chemistry and Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, PO Box 124, SE-221 00 Lund, Sweden.

Analytical Chemistry
|January 15, 2004
PubMed
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Acoustic levitation enables miniaturized biospecific affinity separations in a single drop. This technique efficiently separates biotinylated liposomes using polymer two-phase systems for potential single-cell analysis.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biotechnology

Background:

  • Miniaturization in biological separations presents challenges due to unfavorable surface-area-to-volume ratios.
  • Acoustic levitation offers a unique approach to minimize surface adsorption issues in microscale liquid handling.

Purpose of the Study:

  • To describe a miniaturized (<1 microL) biospecific affinity two-phase partitioning method using acoustically levitated drops.
  • To demonstrate the separation of biotinylated liposomes from a polymer two-phase system within a levitated drop.

Main Methods:

  • Acoustic levitation was used to trap and manipulate aqueous poly(ethylene glycol)/dextran two-phase drops.
  • Phase mixing and separation were controlled by adjusting the ultrasonic field.
  • NeutrAvidin-dextran served as the affinity ligand for biotinylated liposomes.

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Main Results:

  • Biospecific affinity partitioning was successfully achieved in a single acoustically levitated drop.
  • Biotinylated liposomes were effectively redistributed from the poly(ethylene glycol)-rich phase to the dextran-rich phase.
  • The entire separation procedure, including mixing, incubation, and phase separation, was performed within the levitated drop.

Conclusions:

  • Acoustic levitation provides a novel platform for miniaturized, high-efficiency affinity separations.
  • This technique minimizes adsorption issues inherent in microscale liquid handling.
  • The method holds potential for analyzing biologically active components from individual cells.