Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Development and characterization of a conditional mitochondrial complex I assembly system.

Nagendra Yadava1, Toby Houchens, Prasanth Potluri

  • 1Section of Molecular Biology, Division of Biological Sciences, University of California-San Diego, La Jolla, CA 92093-0322, USA.

The Journal of Biological Chemistry
|January 15, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mitochondrial OXPHOS restricts SARS-CoV-2 replication.

Science advances·2026
Same author

Author Correction: Partial restoration of mitochondrial dysfunction by AAV-Ant1 protects from dilated cardiomyopathy in Ant1<sup>-/-</sup> plus mtDNA mutant mice.

Nature communications·2026
Same author

Partial restoration of mitochondrial dysfunction by AAV-Ant1 protects from dilated cardiomyopathy in Ant1<sup>-/-</sup> plus mtDNA mutant mice.

Nature communications·2025
Same author

Idebenone Enhances the Early Microglial Response to Traumatic Brain Injury and Mitigates Acute Gene Expression Changes to Ephrin-A and Dopamine Signaling Pathways.

bioRxiv : the preprint server for biology·2025
Same author

Idebenone Mitigates Traumatic-Brain-Injury-Triggered Gene Expression Changes to Ephrin-A and Dopamine Signaling Pathways While Increasing Microglial Genes.

Cells·2025
Same author

Mitochondrial antioxidants abate SARS-COV-2 pathology in mice.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same journal

Wanted and unwanted modifications of mRNA, and their effect on gene expression and signaling.

The Journal of biological chemistry·2026
Same journal

TGF-β2 drives lipid droplet accumulation in chondrocytes through the TβRI/p-Smad3/Fabp5 axis.

The Journal of biological chemistry·2026
Same journal

Macrophage-specific targeting of histone demethylases with small-molecule inhibitors suppresses inflammatory response in vivo.

The Journal of biological chemistry·2026
Same journal

Substrate and target selectivity of 4'-fluoroadenosine against viral and host polymerases.

The Journal of biological chemistry·2026
Same journal

Correction: Characterization of Mast2 kinase defines structural features, regulation, and substrates.

The Journal of biological chemistry·2026
Same journal

Isotope-Edited ESEEM: A New Method for Probing Copper Binding Sites in Neurodegenerative Proteins.

The Journal of biological chemistry·2026
See all related articles

Researchers created a novel system to study mitochondrial complex I assembly. This system highlights the critical role of the MWFE protein in complex I biogenesis and cellular energy production.

Area of Science:

  • Mitochondrial biology
  • Cellular metabolism
  • Biochemistry

Background:

  • Mitochondrial complex I is crucial for cellular respiration and ATP production.
  • The assembly of complex I is a complex process involving numerous subunits.
  • The specific roles of some complex I subunits, like MWFE, are not fully understood.

Purpose of the Study:

  • To develop a conditional system for studying complex I assembly.
  • To investigate the role of the NDUFA1 gene (encoding MWFE protein) in complex I biogenesis.
  • To explore the relationship between MWFE, mitochondrial DNA-encoded subunits, and complex I activity.

Main Methods:

  • Development of a doxycycline-inducible system in a Chinese hamster fibroblast mutant (CCL16-B2) lacking NDUFA1.
  • Expression of hemagglutinin (HA) epitope-tagged MWFE protein under doxycycline control.

Related Experiment Videos

  • Analysis of complex I assembly, protein stability, and mitochondrial protein synthesis.

    • Main Results:

    • The conditional system allowed precise control over MWFE expression, demonstrating its essential role in complex I assembly.
    • MWFE protein reached steady-state levels within 24 hours of induction, but active complex I assembly took an additional ~24 hours.
    • MWFE was found in a precomplex with mitochondrial DNA-encoded subunits, and its stability was linked to mitochondrial protein synthesis.

    Conclusions:

    • MWFE is a critical component in the pathway of complex I assembly.
    • Complex I assembly is tightly regulated and dependent on mitochondrial protein synthesis and mtDNA-encoded subunits.
    • The developed conditional system offers a valuable tool for studying mitochondrial function, energy metabolism, and reactive oxygen species production.