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Related Experiment Videos

Gold-coated microelectrode array with thiol linked self-assembled monolayers for engineering neuronal cultures.

Yoonkey Nam1, John C Chang, Bruce C Wheeler

  • 1Department of Electrical and Computer Engineering, Beckman Institute, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. ynaml@uiuc.edu

IEEE Transactions on Bio-Medical Engineering
|January 16, 2004
PubMed
Summary

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Researchers developed a gold coating for microelectrode arrays (MEAs) to improve neuronal network control and recording. This surface modification enhances in vitro neuronal circuit studies and potential neural prosthetics.

Area of Science:

  • Neuroscience
  • Materials Science
  • Bioengineering

Background:

  • Microelectrode arrays (MEAs) are crucial for studying neuronal activity.
  • Surface modification of MEAs is challenging but essential for controlling neuronal network formation.
  • Alkanethiol self-assembled monolayers (SAMs) offer reliable surface chemistry but require specific substrates.

Purpose of the Study:

  • To develop a method for applying alkanethiol SAM chemistry to MEAs.
  • To investigate the effect of gold coating on MEA performance for neuronal recordings.
  • To control the geometry of in vitro neuronal networks using surface modifications.

Main Methods:

  • Depositing a thin gold film with a titanium adhesive layer onto planar MEAs.
  • Utilizing polylysine linked to alkanethiol SAMs for surface patterning.

Related Experiment Videos

  • Recording neuronal action potentials from cultured hippocampal networks on modified MEAs.

    • Main Results:

    • The gold coating enabled the application of alkanethiol SAM chemistry to MEAs.
    • Polylysine-functionalized SAMs successfully controlled the geometry of in vitro neuronal networks.
    • Neuronal recordings showed no significant alteration of electrode properties due to the gold coating.

    Conclusions:

    • Gold-coated MEAs provide a versatile platform for advanced neuronal network engineering.
    • This technique facilitates the study of in vitro neuronal circuits for neuroscience research.
    • The approach holds promise for developing neural prostheses and cell-based biosensors.