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Related Experiment Videos

Bioaugmentation for nitrification at cold temperatures.

M A Head1, J A Oleszkiewicz

  • 1Civil Engineering, University of Manitoba, Winnipeg, MB, Canada R3T 5V6. umheadma@cc.umanitoba.ca

Water Research
|January 16, 2004
PubMed
Summary
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Bioaugmentation with nitrifying bacteria can enhance wastewater treatment in cold conditions. Seeding bacteria acclimated at warmer temperatures into cold sequencing batch reactors (SBRs) achieved full ammonia removal, even below the minimum solids retention time.

Area of Science:

  • Environmental microbiology
  • Wastewater treatment engineering
  • Bioreactor design

Background:

  • Short solids retention time (SRT) nitrification is crucial for upgrading wastewater treatment plants, especially in cold climates.
  • Dewatering liquor from anaerobically digested sludge offers a potential ammonia source for nitrifying bacteria production.
  • Bioaugmentation is a viable strategy to improve nitrification efficiency under challenging conditions.

Purpose of the Study:

  • To assess the impact of sudden temperature decreases on nitrification rates.
  • To evaluate the efficacy of seeding cold sequencing batch reactors (SBRs) with nitrifying bacteria acclimated to warmer temperatures (20°C).
  • To determine if nitrification can be achieved at 10°C using bioaugmentation.

Main Methods:

Related Experiment Videos

  • Nitrifying bacteria, cultivated using dewatering liquor at 20°C, 25°C, and 30°C, were used as inocula for cold SBRs (10°C).
  • SBRs were operated at various hydraulic retention times (43.3 to 96 hours).
  • The study monitored nitrification rates and ammonia nitrogen removal efficiency under cold conditions.

    • Main Results:

    • Cooling biomass from 20°C, 25°C, and 30°C to 10°C resulted in average nitrification rate decreases of 58%, 71%, and 82%, respectively.
    • Full ammonia nitrogen removal was achieved in cold SBRs, even when operated at apparent solids retention times (SRTs) below the minimum required (SRT(min)).
    • Bioaugmentation allowed for successful nitrification despite significant temperature drops.

    Conclusions:

    • Wastewater bioaugmentation with pre-acclimated nitrifying bacteria is effective for cold-temperature nitrification in SBRs.
    • Nitrification can be successfully accomplished at 10°C by seeding with bacteria acclimated to 20°C, demonstrating resilience to temperature shock.
    • This approach enables efficient ammonia removal in cold climates and during plant upgrades, even at operational SRTs below SRT(min).