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[Amplification and cloning of the N gene of SARS-associated coronavirus]

Wei-wei Xiao¹, Wen-li Ma, Bao Zhang

¹Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.

Di 1 Jun Yi Da Xue Xue Bao = Academic Journal of the First Medical College of PLA

|January 16, 2004

Abstract

Objective:

To amplify and clone the N gene of severe acute respiratory syndrome-associated coronavirus.

Method:

Using primer Premier 5.0 software, two pairs of nested PCR primers were designed to amplify the N gene. After purification, the amplified products were cloned into pMD18-T vectors, and the positive clones with the inserted fragments were identified by sequence analysis.

Results:

The amplified products was about 1 375 bp in length, and sequence analysis demonstrated that the N gene fragments had been successfully inserted into pMD18-T vectors.

Conclusion:

The successful amplification and cloning of N gene facilitates further investigation of the expression of the N protein and study of its structure and functions.

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