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Updated: Aug 29, 2026
Isolation of Primary Human Colon Tumor Cells from Surgical Tissues and Culturing Them Directly on Soft Elastic Substrates for Traction Cytometry
Published on: June 4, 2015
[Electron microscopic observation of primary cultured laterally spreading tumor cell line]
Yu-jing Han1, Zhuo-sheng Lai, Ya-dong Wang
1Institute for Digestive Diseases of PLA, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To observe the microscopic characteristics of laterally spreading tumor (LST) cell line in primary culture.
Methods:
The cells isolated from a rectum LST specimen obtained by endoscopic mucosal resection was primary cultured, followed by observation with scanning and transmission electron microscope in comparison with the cells of adenocarcinoma and normal mucosa of the rectum.
Results:
Scanning and transmission electron microscopes both revealed numerous microvilli covering the surface of the LST cells, and the cytoplasm contained large quantity of lysosomes, mitochondria and phagosomes. Obviously heterogeneous cell nuclei were present with abnormal nuclear fossa and huge nucleoli.
Conclusion:
The cultured LST cells are highly malignant.
