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Updated: Aug 29, 2026
An Efficient Method for Adenovirus Production
Published on: June 10, 2021
[An easier method for generating recombinant adenovirus containing CD/TK fusion gene driven by vascular endothelial
Yuan-yuan Huang1, Zong-hai Huang, Jian-fa Chen
1Department of General Surgery, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China.
Objective:
To efficiently construct the recombinant adenovirus containing CD/TK fusion gene driven by vascular endothelial growth factor (VEGF) promoter using improved homologous recombination in bacteria.
Methods:
Chemical transformation, instead of electroporation, of the plasmid pAdEasy-1 into E.coli BJ5183 strain was performed to prepare BJ5183pAdEasy-1 as the competent bacterium, in which pAdEasy-1 and pAdtrack-VEGFP-CDglyTK were recombined. Finally, the recombinant adenovirus of AdVEGFP-CDglyTK was constructed by transfecting 293 cells with linearized adenoviral genomes of pAdEasy-VEGFP-CDglyTK.
Results:
This new transformation procedure generated recombinant plasmids in a yield of 60% (6/10), and the adenovirus AdVEGFP-CDglyTK was harvested 7-12 d after transfection.
Conclusion:
The improved homologous recombination in bacteria is efficient, convenient and easy to be carried out.
