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A rearranged junD transforms chicken embryo fibroblasts
1Department of Microbiology, University of Southern California School of Medicine, Los Angeles 90033.
Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|December 1, 1992
Summary
The chicken junD gene, when modified with its untranslated regions and inserted into a retroviral vector, generated a transforming virus. This virus
Area of Science:
- Molecular Biology
- Virology
- Oncology
Background:
- The JunD protein is a transcription factor involved in cellular processes.
- Retroviral vectors are commonly used for gene delivery and studying gene function.
Purpose of the Study:
- To investigate the transforming potential of the chicken junD gene.
- To identify the specific domains of JunD responsible for transformation and transactivation.
Main Methods:
- Synthesis of complementary DNA from chicken junD mRNA.
- Insertion into the RCAS retroviral expression vector.
- Transfection of chicken embryo fibroblast cultures.
- Analysis of viral genomes and protein products.
- Transactivation assays of the collagenase promoter.
Main Results:
- A construct containing the full-length junD mRNA (JD) induced cell transformation.
- The recovered virus (JDV) exhibited moderate transforming and weak oncogenic activity.
- Genomic rearrangement in JDV led to a Gag-JunD fusion protein, responsible for transformation.
- Duplication of an amino-terminal JunD domain was crucial for transformation and transactivation.
Conclusions:
- The Gag-JunD fusion protein is the effector of transformation.
- Duplication of an amino-terminal JunD domain is essential for JunD-mediated transformation and transactivation.