Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human adipose cells have voltage-dependent potassium currents.

M P Ramírez-Ponce1, J C Mateos, J A Bellido

  • 1Dpto. De Fisiología Médica y Biofísica, Facultad de Medicina, Avda. Sánchez Pizjuán 4, 41009 Sevilla, Spain. pponce@us.es

The Journal of Membrane Biology
|January 16, 2004
PubMed
Summary

Human adipose cells possess voltage-dependent potassium currents, crucial for their electrical properties. These findings reveal specific characteristics of potassium channels in these cells.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Energy Spectrum of Ultrahigh-Energy Cosmic Rays across Declinations -90° to +44.8° as Measured at the Pierre Auger Observatory.

Physical review letters·2026
Same author

Search for the Anomalous Events Detected by ANITA Using the Pierre Auger Observatory.

Physical review letters·2025
Same author

Demonstrating Agreement between Radio and Fluorescence Measurements of the Depth of Maximum of Extensive Air Showers at the Pierre Auger Observatory.

Physical review letters·2024
Same author

Limits to Gauge Coupling in the Dark Sector Set by the Nonobservation of Instanton-Induced Decay of Super-Heavy Dark Matter in the Pierre Auger Observatory Data.

Physical review letters·2023
Same author

Measurement of the Fluctuations in the Number of Muons in Extensive Air Showers with the Pierre Auger Observatory.

Physical review letters·2021
Same author

Features of the Energy Spectrum of Cosmic Rays above 2.5×10^{18}  eV Using the Pierre Auger Observatory.

Physical review letters·2020

Area of Science:

  • Cellular Electrophysiology
  • Adipocyte Biology
  • Ion Channel Physiology

Background:

  • Human adipocytes play roles in metabolism and energy storage.
  • Understanding adipocyte membrane properties is key to metabolic research.
  • Specific ion channel functions in human adipose tissue remain incompletely characterized.

Purpose of the Study:

  • To investigate the membrane electrical properties of human adipocytes.
  • To identify and characterize voltage-gated ion channels in these cells.
  • To determine the specific role of potassium currents in human adipose cells.

Main Methods:

  • Whole-cell patch-clamp technique applied to differentiated human adipocytes.
  • Analysis of outward currents, activation kinetics, and inactivation properties.

Related Experiment Videos

  • Ion selectivity assays and pharmacological blockades using tetraethylammonium and barium.

    • Main Results:

    • Differentiated human adipocytes exhibit outward currents with sigmoidal activation kinetics.
    • These currents activate between -20 to -30 mV and show slow inactivation.
    • Channels are highly K(+)-selective, with permeabilities K(+)>NH4+>Cs(+)>Na(+), and are blocked by tetraethylammonium and barium.

    Conclusions:

    • Human adipose cells possess functional voltage-dependent potassium currents (I(KV)).
    • These currents are mediated by highly K(+)-selective channels.
    • The findings contribute to understanding the electrophysiological basis of human adipocyte function.