Alteration of cytochrome P-450 2C11-dependent testosterone metabolism in Gunn rat liver

Cytochrome P-450 2C11 (CYP2C11) is the main isozyme present in adult male rat liver and specifically hydroxylates testosterone in positions 2 alpha and 16 alpha. In Gunn rats, this isozyme is recognized by the anti-CYP2C11 antibody but its activity towards testosterone is dramatically decreased. Moreover, peptide mapping, after digestion of microsomal fractions by V8 protease and probing with anti-CYP2C11 antibody, exhibit a pattern which differs from that obtained with Wistar rats. Taken together, data suggest that the protein sequence of CYP2C11 from the Gunn rat differs from that of the Wistar rat.