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Updated: Aug 29, 2026
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Published on: June 6, 2016
NF-kappaB activation at implantation window of the mouse uterus
Hitomi Nakamura1, Tadashi Kimura, Kazuhide Ogita
1Division of Obstetrics and Gynecology, Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan.
Problem:
Nuclear factor kappa B (NF-kappaB) is one candidate transcriptional modulator, which might regulate many kinds of molecules that play sequential roles at implantation in the endometrium. However, temporal and spatial activation of NF-kappaB at implantation window is unknown.
Methods:
Activation of NF-kappaB in the mouse uterus was determined by electrophoretic mobility shift assays. Localization of p50 and p65, components of NF-kappaB, was analyzed by immunohistochemistry.
Results:
NF-kappaB was activated in the proestrus and estrus phases in non-pregnant uterus. In the pregnant uterus, NF-kappaB was activated after day 1.5 post-coitum, and the activation continued during implantation period. The immunoreactivities of p50 and p65 were mainly localized in endometrial epithelium, and were weaker in endometrial stroma cells.
Conclusion:
NF-kappaB activity is dynamically regulated during the sexual cycle as well as during the implantation period in the endometrium, where the biochemical interaction between mother and conceptus first occurs.
