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Related Concept Videos

Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Stem Cell Therapy for Tissue Regeneration01:21

Stem Cell Therapy for Tissue Regeneration

Stem cell therapy is a method used in regenerative medicine to repair and restore function to damaged tissues and organs. Stem cells have the potential to proliferate and differentiate into various tissue types, making them ideal candidates for tissue regeneration. For example, hematopoietic stem cell transplants are commonly used in blood cancer treatment to replenish damaged bone marrow and restore healthy blood cells.
Types of Stem Cells used in Stem Cell Therapy
The two main cell types that...
Production of Formed Elements01:34

Production of Formed Elements

Hemangioblasts are multipotent stem cells originating from the mesoderm. They give rise to hematopoietic stem cells (HSCs), which undergo hematopoiesis to produce all the formed elements of blood. This process is regulated by a complex network of hematopoietic growth factors, including transcription factors, growth factors, and cytokines. These factors stimulate the HSCs to divide and differentiate, though some HSCs remain undifferentiated to maintain a self-renewing pool.
Most HSCs commit to...

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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness
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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

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SCL is required for normal function of short-term repopulating hematopoietic stem cells.

David J Curtis1, Mark A Hall, Leonie J Van Stekelenburg

  • 1Rotary Bone Marrow Research Laboratory, Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia. dcurtis@wehi.edu.au

Blood
|January 17, 2004
PubMed
Summary

The stem cell leukemia (SCL) gene is crucial for adult hematopoietic stem cells (HSCs). Deleting SCL increases HSC numbers but severely impairs their repopulation ability, indicating SCL

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Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells
06:41

Isolation Method for Long-Term and Short-Term Hematopoietic Stem Cells

Published on: May 19, 2023

Area of Science:

  • Hematology
  • Molecular Biology
  • Developmental Biology

Background:

  • The stem cell leukemia (SCL) gene is vital for embryonic hematopoietic stem cell (HSC) development.
  • Its role in adult HSCs remains incompletely understood.

Purpose of the Study:

  • To investigate the function of the SCL gene in adult hematopoietic stem cells (HSCs) using a conditional gene targeting approach.
  • To determine the impact of SCL deletion on HSC number, repopulation capacity, and self-renewal.

Main Methods:

  • Conditional gene targeting to delete the SCL gene in adult mouse hematopoietic stem cells.
  • Flow cytometry analysis of bone marrow cells to quantify phenotypic HSCs (Linneg c-kit+ Sca-1+).
  • Competitive repopulation assays and secondary transplantation to assess HSC function and self-renewal.

Main Results:

  • SCL deletion led to a 4-fold increase in the number of phenotypic HSCs.
  • Despite increased numbers, SCL-deleted bone marrow cells exhibited a severe multilineage defect in repopulation capacity.
  • SCL-heterozygous cells showed a mild repopulation defect, suggesting haploinsufficiency.
  • The transplantation defect was evident early (within 4 weeks) and affected multipotent progenitors or short-term repopulating HSCs.
  • SCL appears not to be required for HSC self-renewal, as defects stabilized in secondary transplants.
  • Generating SCL-deleted cells within SCL-wild-type mice rescued the early repopulating defect.

Conclusions:

  • SCL is essential for the normal function of short-term repopulating HSCs.
  • Haploinsufficiency of SCL also impacts HSC repopulation capacity.
  • SCL's primary role in adult hematopoiesis is linked to progenitor function rather than HSC self-renewal.