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Genetic diversity within Pisum sativum using protein- and PCR-based markers
A Baranger1, G Aubert, G Arnau
1Domaine de la Motte, UMR INRA-ENSAR APBV, BP35327, 35653 Le Rheu, France. baranger@rennes.inra.fr
Summary
Molecular markers revealed pea (Pisum sativum) breeding lineages in Western Europe, tracking traits like seed weight and frost tolerance. This study highlights the vast genetic diversity within peas, despite narrow recent cultivar bases.
Area of Science:
- Agricultural Science
- Genetics
- Plant Breeding
Background:
- Pea (Pisum sativum) germplasm diversity is crucial for crop improvement.
- Understanding historical breeding trends and objectives is essential for future research.
Purpose of the Study:
- To classify Pisum accessions using molecular markers.
- To trace major pea breeding lineages and objectives in Western Europe.
- To assess genetic diversity and propose a core collection.
Main Methods:
- Utilized 121 protein- and PCR-based molecular markers.
- Analyzed 148 Pisum accessions from Western Europe.
- Compared molecular classification with existing pedigree data.
Main Results:
- Successfully structured pea accessions, distinguishing varietal types (fodder, food, feed) and wild forms.
- Identified distinct gene pools for winter-sown feed peas.
- Observed a paradox between narrow genetic base of recent cultivars and broad overall diversity.
- Proposed a 43-accession sub-collection capturing 96% of allelic variability.
Conclusions:
- Molecular markers effectively reconstruct pea breeding history and varietal differentiation.
- Significant genetic diversity exists within Pisum sativum, offering potential for future breeding.
- A core collection is proposed to efficiently utilize this diversity.