[A study of aspermia-related genes by genchips and analysis of the RAP1A gene]
Bo Yang1, Xiao-Kang Gao, He Wang
1Department of Urology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi 710032, China.
Objective:
To study the differential gene expression profiles between the normal and aspermia human testes by genechips.
Methods:
Probes were prepared from mRNA extracted from both normal and aspermia testes and employed on Biostar H-40s genechips to detect the differential gene expression profiles. A distinctly up-regulated gene RAP1A was analyzed by bibliogrphic retrieval.
Results:
Six hundred and twenty-three differential expressed genes were found, among which the distinctly up-regulated gene RAP1A was closely related to human sperm regulation.
Conclusions:
Screening the differential gene expression profiles between the normal and aspermia human testes by genechips can be used in the study of aspermia-related genes.
