Related Experiment Video
Updated: Aug 29, 2026
Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
[Protein and mRNA expression of nitric oxide synthase isoforms I and III in rat penile spongium tissues]
Jun-Ping Shi1, Yong-Mei Zhao, Gang Qi
1Department of Andrology, Shenyang Andrology Hospital, Shenyang, Liaoning 110032, China.
Objective:
To investigate protein and mRNA expression of nitric oxide synthase (NOS) isoforms I and III in rat penile tissues.
Methods:
Penile tissues from male SD rats were investigated. The protein expression of NOS I and III in rat penis was confirmed by immunoprecipitation and Western blot. Gene expression of both NOS I and III was identified by reverse transcription-polymerase chain reaction.
Results:
The mRNA and protein products of NOS I and III are both expressed in normal rat penile spongium tissues. But, NOS III mRNA and protein products are more highly expressed than those of NOS.
Conclusions:
These findings support the possibility that penile erection is regulated by different NOS isoforms in rat penile tissues.
