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Updated: Aug 29, 2026
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
Published on: January 17, 2014
Utilizing three-dimensional fluorescence's red-shift cascade effect to monitor mycobacterium PRY-1 degradation of
Joseph F Sinski1, Bradley S Compton, B Shea Perkins
1Bellarmine University, Department of Chemistry, 2001 Newberg Road, Louisville, Kentucky 40205, USA. jsinski@bellarmine.edu
Abstract:
Samples of Mycobacterium PRY-1 inoculated motor oil are subjected to three-dimensional fluorescence spectroscopy to document the shifting of excitation/emission maxima as the solutions undergo serial dilutions. Effects such as self-quenching of individual polycyclic aromatic hydrocarbons (PAHs) and energy transfer between PAHs combine to produce large red-shifts in the resulting fluorescence emission spectra. This process is repeated over a series of weeks and is compared to preceding spectra to gauge the microbial degradation of the petroleum. Results show a two-fold, or 75%, PAH contaminant degradation by Mycobacterium PRY-1 over a 140-day growth period.
