Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Bone Remodeling

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Release of a wound-healing agent from PLGA microspheres in a thermosensitive gel.

BioMed research international·2013

Same author

Antimicrobial peptides for plaque control.

Advances in dental research·2009

Same author

In vitro cytotoxicity of mitoxantrone-incorporated albumin microspheres on acute promyelocytic leukaemia cells.

Journal of microencapsulation·2005

Same author

A novel in vitro release technique for peptide containing biodegradable microspheres.

AAPS PharmSciTech·2004

Same author

Preparation, characterization, and in vivo evaluation of salmon calcitonin microspheres.

AAPS PharmSciTech·2004

Same author

Effect of a freeze-dried CMC/PLGA microsphere matrix of rhBMP-2 on bone healing.

AAPS PharmSciTech·2004

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

Porous bone morphogenetic protein-2 microspheres: polymer binding and in vitro release.

J A Schrier¹, P P DeLuca

¹Faculty of Pharmaceutical Sciences, College of Pharmacy, University of Kentucky, Lexington, KY 40536, USA.

AAPS Pharmscitech

|January 20, 2004

Summary

Hydrophilic poly-lactic-co-glycolic acid (PLGA) microspheres enhance recombinant human bone morphogenetic protein 2 (rhBMP-2) binding. PLGA polymer properties and adsorption techniques control rhBMP-2 release for effective delivery.

More Related Videos

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Published on: May 16, 2022

Related Experiment Videos

Last Updated: Jul 15, 2026

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Polyelectrolyte Complex for Heparin Binding Domain Osteogenic Growth Factor Delivery

Published on: August 22, 2016

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Site-Directed Immobilization of Bone Morphogenetic Protein 2 to Solid Surfaces by Click Chemistry

Published on: March 29, 2018

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Fabricating Highly Open Porous Microspheres (HOPMs) via Microfluidic Technology

Published on: May 16, 2022

Area of Science:

Biomaterials Science
Drug Delivery Systems
Tissue Engineering

Background:

Recombinant human bone morphogenetic protein 2 (rhBMP-2) is crucial for bone regeneration.
Poly-lactic-co-glycolic acid (PLGA) microspheres are widely used for controlled protein delivery.
Optimizing rhBMP-2 binding and release from PLGA carriers is essential for therapeutic efficacy.

Purpose of the Study:

To compare the binding and release characteristics of rhBMP-2 with hydrophobic and hydrophilic PLGA copolymers.
To investigate the influence of PLGA properties and adsorption techniques on rhBMP-2 delivery.
To establish control over rhBMP-2 release profiles through PLGA microsphere carrier selection.

Main Methods:

Porous PLGA microspheres were fabricated using a double emulsion method.
rhBMP-2 binding was quantified by measuring protein concentration after soaking microspheres.
Protein release kinetics were analyzed over time using reversed-phase high-performance liquid chromatography.

Main Results:

Hydrophilic PLGA microspheres exhibited significantly higher rhBMP-2 binding capacity compared to hydrophobic counterparts.
Increased binding correlated with higher polymer acid values; adsorption was influenced by protein concentration and technique.
rhBMP-2 release occurred in two phases: rapid release of unbound protein within 3 days, followed by sustained release tied to PLGA mass loss.

Conclusions:

PLGA microsphere properties, particularly hydrophilicity and acid value, critically influence rhBMP-2 binding.
Adsorption techniques and protein concentration can be optimized to enhance rhBMP-2 loading.
Selection of appropriate PLGA microsphere carriers allows for controlled delivery of rhBMP-2.