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Updated: Aug 18, 2026
Measuring Influenza Neutralizing Antibody Responses to A(H3N2) Viruses in Human Sera by Microneutralization Assays Using MDCK-SIAT1 Cells
Published on: November 22, 2017
Decreased measles virus-neutralizing activity in sera from otosclerotic patients
Stephan Lolov1, Petya Zarzalanova, George Edrev
1Department of Molecular Immunology, Institute of Biology and Immunology, Bulgarian Academy of Sciences, Sofia, Bulgaria. Stephan@lolov.com
Hypothesis:
Any kind of depressed systemic anti-measles reaction can lead to the induction of a local immune response in the inner ear and possibly to reactivation of bone turnover in this region.
Methods:
Different dilutions of sera were tested for neutralizing activity against a constant viral concentration. The ability of measles virus to infect and replicate in the cell monolayer was detected by enumeration of living and growing cells with a colored reaction.
Results:
Virus-neutralizing activity in the sera of patients with confirmed otosclerosis was significantly weaker than in that of healthy controls. When age- and sex-matched pairs were compared, the neutralizing activity in the healthy counterpart was higher in 5 cases. Nearly complete viral neutralization was achieved with samples containing inactivated complement and in IgG-containing fractions, but not in immunoglobulin-depleted samples.
Conclusion:
The present study is consistent with measles virus participation at least in the initiation of some cases of otosclerosis.
