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Related Experiment Videos

Destruction with a box: substrate recognition by the anaphase-promoting complex.

Wolfgang Zachariae1

  • 1Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany.

Molecular Cell
|January 21, 2004
PubMed
Summary

Destruction boxes directly bind to the anaphase-promoting complex (APC/C) during mitosis. This interaction is cell cycle-regulated and does not require Cdc20 activators, challenging previous understanding of substrate recruitment.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The anaphase-promoting complex (APC/C) is a crucial ubiquitin ligase regulating cell cycle progression.
  • Protein degradation via ubiquitination, mediated by the APC/C, is essential for accurate mitosis.
  • Destruction boxes (D-boxes) on target proteins signal for APC/C-mediated degradation.

Discussion:

  • Yamano et al. demonstrate a direct physical interaction between destruction boxes and the APC/C.
  • This direct binding occurs in a cell cycle-dependent manner, suggesting a dynamic regulatory mechanism.
  • The study reveals that APC/C activators, such as Cdc20, are not essential for this substrate recruitment step.

Key Insights:

  • Direct binding of destruction boxes to the APC/C is a key mechanism for substrate recognition.

Related Experiment Videos

  • Cell cycle regulation of D-box/APC/C interaction controls protein degradation timing.
  • The requirement for Cdc20 family activators in substrate recruitment is re-evaluated.

    • Outlook:

    • Further investigation into the structural basis of D-box/APC/C interaction.
    • Exploring alternative APC/C activators or pathways involved in substrate targeting.
    • Understanding the implications for cell cycle control and potential therapeutic interventions in cancer.