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Related Experiment Videos

The coiled body.

A I Lamond1, M Carmo-Fonseca

  • 1European Molecular Biology Laboratory, Meyerhofstrasse 1, Postfach 102209, D6900 Heidelberg, Germany.

Trends in Cell Biology
|June 1, 1993
PubMed
Summary
This summary is machine-generated.

The coiled body, a nuclear organelle, contains key splicing factors and is conserved across species. This dynamic structure assembles and disassembles, potentially acting as a distinct nuclear metabolic compartment.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • The coiled body is a nuclear organelle crucial for cellular processes.
  • It contains essential splicing factors like snRNPs and U2AF, and nucleolar protein fibrillarin.
  • This organelle is evolutionarily conserved in both animal and plant cells.

Purpose of the Study:

  • To elucidate the composition and dynamic nature of the coiled body.
  • To understand the role of the coiled body in nuclear organization and function.
  • To investigate the potential of the coiled body as a distinct nuclear metabolic compartment.

Main Methods:

  • Immunofluorescence microscopy to visualize coiled body components.
  • Biochemical fractionation to isolate and analyze coiled body contents.

Related Experiment Videos

  • Cellular imaging techniques to observe dynamic assembly and disassembly cycles.

    • Main Results:

    • Confirmed the presence of snRNPs, U2AF, and fibrillarin within the coiled body.
    • Demonstrated conserved structure and composition across diverse species.
    • Observed regulated cycles of coiled body assembly and disassembly during interphase and mitosis.

    Conclusions:

    • The coiled body is a fundamental nuclear organelle with a conserved role in splicing.
    • Its dynamic nature suggests active regulation within the nucleus.
    • The coiled body may function as a specialized metabolic compartment within the nucleus.