Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

TGN38/41: a molecule on the move.

K K Stanley1, K E Howell

  • 1Heart Research Institute, 145 Missenden Road, Camperdown, Sydney, NSW 2050, Australia.

Trends in Cell Biology
|August 1, 1993
PubMed
Summary

Trans-Golgi Network protein 38/41 (TGN38/41) is crucial for exocytic vesicle formation. It acts as a receptor, linking secretory proteins to vesicle budding at the TGN.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of multiplex tandem PCR (MT-PCR) assays for the detection of bacterial resistance genes among Enterobacteriaceae in clinical urines.

The Journal of antimicrobial chemotherapy·2018
Same author

Constructing expression cDNA libraries using unphosphorylated adaptors.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Expression Screening of cDNA Libraries in pEX.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Epitope Mapping Using pEX.

Methods in molecular biology (Clifton, N.J.)·2011
Same author

Interaction of caveolin with 7-ketocholesterol.

Atherosclerosis·2001

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • TGN38/41 is a heterodimeric integral membrane protein.
  • It cycles between the trans-Golgi network (TGN) and the cell surface.
  • A specific tyrosine-containing motif in its tail dictates its TGN localization.

Purpose of the Study:

  • To investigate the role of TGN38/41 in exocytic vesicle formation.
  • To elucidate the mechanism by which TGN38/41 mediates vesicle budding.
  • To explore the potential coupling of secretory protein segregation with vesicle budding.

Main Methods:

  • The study likely involved protein localization studies and biochemical assays.
  • Investigated the interaction between TGN38/41, p62, and rab GTPases.
  • Examined the necessity of the TGN38/41 cytoplasmic domain for complex binding and budding.

Main Results:

  • TGN38/41 is essential for exocytic vesicle formation at the TGN.
  • It functions as a receptor for complexes involving p62 and rab GTPases (e.g., rab6).
  • Binding of this complex to the TGN38/41 cytoplasmic domain is required for vesicle budding.

Conclusions:

  • TGN38/41 plays a critical role in the budding of exocytic vesicles from the TGN.
  • The protein likely couples the sorting of secretory proteins with the physical process of vesicle formation.
  • This mechanism highlights TGN38/41's importance in the secretory pathway.

Related Experiment Videos