Inhibition of sphingolipid synthesis: effects on glycosphingolipid-GPI-anchored protein microdomains

The idea that the transport and sorting of glycosylphosphatidylinositol (GPI)-anchored proteins depends on their interaction with glycosphingolipids was first proposed five or six years ago. Until recently, only circumstantial evidence was available to support this suggestion. During the past year, compelling support for this hypothesis has been provided by observations that inhibition of sphingolipid synthesis reduces the rate of transport of GPI-anchored proteins in yeast, and abolishes the polarized sorting of a GPI-anchored protein in epithelia.