Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tumor necrosis factor alpha disrupts tight junction assembly.

Lisa S Poritz1, Kristian I Garver, Anna F Tilberg

  • 1Department of Surgery, The Milton S. Hershey Medical Center, Hershey, Pennsylvania 17033, USA. lporitz@psu.edu

The Journal of Surgical Research
|January 21, 2004
PubMed
Summary

Tumor necrosis factor-alpha (TNFalpha) disrupts tight junctions (TJs) by reducing claudin-1 levels, potentially increasing intestinal permeability in Crohn

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative host transcriptomic and mucosal microbiome profiling reveals region-specific host-microbiome associations across the human intestine.

bioRxiv : the preprint server for biology·2026
Same author

Loss of STIM2, but not of STIM1, drives colorectal cancer metastasis through metabolic reprogramming and the ATF4 ER stress pathway.

Science signaling·2025
Same author

Apixaban Versus Enoxaparin for Venous Thromboembolism Prophylaxis in Colorectal Surgery.

The Journal of surgical research·2025
Same author

Twenty Years of Disease-Modifying Anti-IBD Drug Use Preceding Colectomy for Refractory IBD-Associated Colitis.

Diseases of the colon and rectum·2025
Same author

Low Rates of Short-Term Anastomotic Complications After Kono-S versus Side-to-Side Stapled Anastomosis.

The Journal of surgical research·2024
Same author

Cystathionine Gamma-Lyase Regulates TNF-α-Mediated Injury Response in Human Colonic Epithelial Cells and Colonoids.

Antioxidants (Basel, Switzerland)·2024

Area of Science:

  • Gastroenterology
  • Cell Biology
  • Immunology

Background:

  • Crohn's disease (CD) is associated with increased intestinal permeability and decreased tight junction (TJ) protein expression.
  • Tumor necrosis factor-alpha (TNFalpha) is implicated in CD pathogenesis, and its suppression offers therapeutic benefits.
  • Key TJ proteins include ZO-1, occludin, and claudins.

Purpose of the Study:

  • To investigate the effect of TNFalpha on TJ structure and protein expression.
  • To determine if TNFalpha-induced TJ disruption is linked to specific TJ protein alterations.

Main Methods:

  • Madin-Darby Canine Kidney (MDCK) cells were treated with varying concentrations of TNFalpha for 5 days.
  • Tight junction structure was assessed using immunofluorescence for ZO-1.

Related Experiment Videos

  • Levels of ZO-1, occludin, and claudin-1 were quantified via western blot.

    • Main Results:

    • TNFalpha qualitatively disrupted TJ structure, evidenced by fragmented ZO-1 staining.
    • No significant changes in ZO-1 or occludin protein levels were observed.
    • A significant decrease in claudin-1 protein was observed with increasing TNFalpha concentrations.

    Conclusions:

    • TNFalpha qualitatively disrupts MDCK cell TJs.
    • This disruption is not due to reduced cell number, confluency, or altered ZO-1/occludin levels.
    • Decreased claudin-1 may cause ZO-1 relocation, contributing to increased intestinal permeability in CD.