Related Experiment Video
Updated: Aug 29, 2026
In Vivo Functional Assessment of Rat Masseter Muscle Following Surgical Creation of a Volumetric Muscle Loss (VML) Injury
Published on: November 15, 2024
[Functional reconstruction with tissue engineered myoblast in facial muscle of rat]
Guilin Huang1, Longjiang Li, Yuming Wen
1Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
Objective:
The purpose of this article is to discuss the reconstruction of facial muscle defects with tissue engineered myoblast in SD rats.
Methods:
Using purified, subcultured myoblast of neonatal rats and type I collagen gels as extracellular matrix (ECM) and scaffold, tissue engineered muscle was transplanted in the face of syngeneic nutured rats.
Results:
Tissue engineered myoblast generated and differentiated in vitro were observed with microscope. Myoblast fused each other and formed myofibers in the face of rats, nerve fibers and vessels regeneration could also be found in some samples. Postoperative electromiographs showed the myofibers were active when stimulating the nerve trunk that innervates the engineered facial muscle.
Conclusion:
Tissue engineered method is hopeful to be used as a new technique to reconstruct defects of facial muscle.
