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Updated: Aug 29, 2026
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
Published on: July 31, 2012
[Expression of constructed eukaryotic vectors carrying encoding gene of soluble human interleukin-1 receptor in
Yan Xu1, Peng Li, Yunhui Zhang
1Department of Oral Medicine, College of Stomatology, Nanjing Medical University, Nanjing 210029, China.
Objective:
This study is to make sure if the constructed pcDNA3 carrying encoding gene of sIL-1R can be expressed in mammalian cells in vitro.
Methods:
COS-1 cells and CHO cells were respectively transfected with recombinant plasmid pcDNA3/sIL-1R by liposome. The protein expression products were detected by ELISA.
Results:
The results indicated that the protein expression products could be detected in the cell plasma and the cell culture supermatant. The expression level in experimental groups was much higher than that in control groups(P < 0.05).
Conclusion:
The constructed pcDNA3/sIL-1R can express interest protein in mammalian cells and this establishes the basis for future investigation on gene therapy of periodonititis and other inflammatory diseases.
