Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Using isotope tracers to study metabolism: application in mouse models.

Brendan J McCabe1, Stephen F Previs

  • 1Department of Nutrition, Case Western Reserve University School of Medicine, 10900 Euclid Avenue Dental Building, Room 201, Cleveland, OH 44106-4906, USA.

Metabolic Engineering
|January 22, 2004
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LC-MS measurement of triglyceride hydrolysis: Application in studies of Patatin-like phospholipase domain-containing protein 3 activity.

Analytical biochemistry·2026
Same author

Microdroplet Mass Spectrometry Enables Rapid and Sensitive Measurement of Glucose Homeostasis for Elucidating Mechanism of Drug Action.

Analytical chemistry·2025
Same author

Elucidation of Mechanism of Action in Drug Invention: Using Stable Isotope Tracers to Unravel Biochemical Kinetics.

Pharmacology research & perspectives·2025
Same author

Quantifying protein kinetics in vivo: influence of precursor dynamics on product labeling.

American journal of physiology. Endocrinology and metabolism·2024
Same author

Author Correction: Functionally selective signaling and broad metabolic benefits by novel insulin receptor partial agonists.

Nature communications·2024
Same author

Functionally selective signaling and broad metabolic benefits by novel insulin receptor partial agonists.

Nature communications·2022

Isotope tracers are valuable tools for studying metabolism in mice, offering insights into carbohydrate, fat, and protein turnover. Labeled water (2H2O and H218O) aids in tracing biological processes, benefiting research into metabolic disorders.

Area of Science:

  • Metabolic research
  • Biochemical analysis
  • Animal models

Background:

  • Isotope tracer methods provide fundamental principles for studying metabolic pathways.
  • Understanding in vivo metabolic regulation is crucial for various physiological and pathological conditions.

Purpose of the Study:

  • To discuss the application of isotope tracers in investigating mouse metabolism.
  • To highlight the use of tracers in studying the regulation of macronutrient turnover.
  • To explore the benefits of labeled water in biological process tracing.

Main Methods:

  • Outlining basic principles of tracer methods.
  • Applying isotope tracers to study carbohydrate, fat, and protein turnover in vivo.
  • Utilizing labeled water (2H2O and H218O) for kinetic analysis.

Related Experiment Videos

Main Results:

  • Demonstrates how isotope tracers elucidate metabolic regulation in mice.
  • Illustrates the utility of labeled water for tracing biological kinetics.
  • Provides background for designing studies on altered metabolic states.

Conclusions:

  • Isotope tracers are effective tools for metabolic research in mice.
  • Labeled water offers advantages for studying biological process kinetics.
  • This approach aids in understanding altered metabolism in conditions like diabetes and cancer cachexia.