Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CpG-DNA aided cross-priming by cross-presenting B cells.

Antje Heit1, Katharina M Huster, Frank Schmitz

  • 1Institute of Medical Microbiology, Immunology and Hygiene, Technical University Munich, Munich, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|January 22, 2004
PubMed
Summary

B cells can cross-present antigens and prime CD8 T cells, similar to dendritic cells (DCs). CpG-DNA conjugation to ovalbumin (OVA) enhances B cell antigen uptake and activation, demonstrating novel B cell functions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Long-term antigen reduction does not achieve durable HBV control in mice but enhances therapeutic vaccine efficacy.

JHEP reports : innovation in hepatology·2026
Same author

The Memory Inflation Response Against Spread-Defective CMV Requires Priming-Independent CD4<sup>+</sup> T Cell Help.

European journal of immunology·2026
Same author

SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cells.

EMBO reports·2026
Same author

SATB1 is a targetable modulator of JAK-STAT signaling and cytokines in human Treg and Tconv cells.

bioRxiv : the preprint server for biology·2026
Same author

Assessing VirScan serosurvey epitope profiling variability between in-clinic venous blood draw and capillary blood self-sampling device.

Microbiology spectrum·2026
Same author

Balancing the efficacy and safety of chimeric antigen receptor T-cell therapy by affinity combination.

Nature communications·2026

Area of Science:

  • Immunology
  • Cell Biology
  • Antigen Presentation

Background:

  • Dendritic cells (DCs) are primarily known for cross-presentation of exogenous antigens via MHC class I to CD8 T cells.
  • CpG-DNA, an immunostimulatory molecule, can enhance antigen presentation when conjugated to antigens like ovalbumin (OVA).

Purpose of the Study:

  • To investigate if B cells can cross-present CpG-OVA complexes and route internalized antigens into the MHC class I presentation pathway.
  • To explore the capacity of B cells to cross-prime naive CD8 T cells.

Main Methods:

  • Conjugation of CpG-DNA to OVA to form CpG-OVA complexes.
  • Assessing OVA internalization by B cells.
  • Analyzing the generation of CD8 T cell epitopes complexed to MHC class I by B cells.

Related Experiment Videos

  • Measuring upregulation of costimulatory molecules and cytokines (e.g., IL-12) in B cells.
  • Adoptive transfer of CpG-OVA complex-loaded B cells into wild-type and MyD88-deficient mice to assess CD8 T cell priming.

    • Main Results:

    • Conjugation of CpG-DNA to OVA enhanced OVA internalization by B cells up to 40-fold.
    • B cells loaded with CpG-OVA complexes generated the SIINFEKL epitope complexed to MHC class I, though less efficiently than DCs.
    • CpG-OVA complex internalization stimulated B cells to upregulate costimulatory molecules and IL-12.
    • Adoptive transfer of CpG-OVA complex-loaded B cells led to cross-priming of naive CD8 T cells in recipient mice.

    Conclusions:

    • B cells possess the capability for cross-presentation of exogenous antigens.
    • B cells can cross-prime naive CD8 T cells, challenging the notion that this is a DC-exclusive function.
    • CpG-DNA conjugation enhances B cell antigen uptake and immune activation, revealing new roles for B cells in adaptive immunity.