Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Possible 110 kDa receptor for interleukin 2 in the chicken.

T H Lee1, C H Tempelis

  • 1Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720.

Developmental and Comparative Immunology
|November 1, 1992
PubMed
Summary

Researchers developed monoclonal antibodies to identify chicken lymphokine receptors on activated lymphocytes. One antibody specifically targets a 110 kDa surface antigen, potentially analogous to the human interleukin 2 receptor gamma subunit.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dispersal, survivorship, and host selection of Culex erythrothorax (Diptera: Culicidae) associated with a constructed wetland in southern California.

Journal of medical entomology·1999
Same author

Critical pathways as a strategy for improving care: problems and potential.

Annals of internal medicine·1995
Same author

Abnormal glucocorticoid receptor-activator protein 1 interaction in steroid-resistant asthma.

The Journal of experimental medicine·1995
Same author

How do cardiologists fit in managed care?

Journal of the American College of Cardiology·1995
Same author

AZT trial in Thailand.

Science (New York, N.Y.)·1995
Same author

Variceal bleeding due to segmental portal hypertension caused by chronic pancreatitis.

The American journal of emergency medicine·1995

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Chicken lymphoblasts are activated lymphocytes crucial for immune responses.
  • Lymphokines mediate communication between immune cells, but their receptors in chickens are not well characterized.

Purpose of the Study:

  • To produce and characterize monoclonal antibodies (mAbs) for identifying chicken lymphokine receptors.
  • To investigate the function and molecular properties of a specific lymphokine receptor on chicken lymphoblasts.

Main Methods:

  • Generation of chicken lymphoblasts via concanavalin A (Con A) stimulation.
  • Production of mAbs by immunizing mice with activated lymphoblasts.
  • Selection of specific mAbs using cellular enzyme immunoassay (EIA) and immunofluorescence staining.

Related Experiment Videos

  • Assessment of mAb inhibitory activity on lymphokine function.
  • Immunoprecipitation to determine the molecular weight of the target antigen.

    • Main Results:

    • A specific mAb was generated that strongly reacted with lymphoblasts but weakly with resting cells, erythrocytes, and bursal cells.
    • This mAb inhibited lymphokine activity in conditioned media from activated spleen cells.
    • Immunoprecipitation identified a lymphoblast surface antigen of approximately 110 kDa.

    Conclusions:

    • A novel monoclonal antibody successfully identified a specific lymphokine receptor on chicken lymphoblasts.
    • The identified 110 kDa receptor may be functionally and structurally analogous to the interleukin 2 receptor gamma subunit in mammals.
    • This research provides a tool for further studying chicken immune cell activation and lymphokine signaling.