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Possible 110 kDa receptor for interleukin 2 in the chicken
1Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720.
Developmental and Comparative Immunology
|November 1, 1992
Summary
Researchers developed monoclonal antibodies to identify chicken lymphokine receptors on activated lymphocytes. One antibody specifically targets a 110 kDa surface antigen, potentially analogous to the human interleukin 2 receptor gamma subunit.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Chicken lymphoblasts are activated lymphocytes crucial for immune responses.
- Lymphokines mediate communication between immune cells, but their receptors in chickens are not well characterized.
Purpose of the Study:
- To produce and characterize monoclonal antibodies (mAbs) for identifying chicken lymphokine receptors.
- To investigate the function and molecular properties of a specific lymphokine receptor on chicken lymphoblasts.
Main Methods:
- Generation of chicken lymphoblasts via concanavalin A (Con A) stimulation.
- Production of mAbs by immunizing mice with activated lymphoblasts.
- Selection of specific mAbs using cellular enzyme immunoassay (EIA) and immunofluorescence staining.
- Assessment of mAb inhibitory activity on lymphokine function.
- Immunoprecipitation to determine the molecular weight of the target antigen.
Main Results:
- A specific mAb was generated that strongly reacted with lymphoblasts but weakly with resting cells, erythrocytes, and bursal cells.
- This mAb inhibited lymphokine activity in conditioned media from activated spleen cells.
- Immunoprecipitation identified a lymphoblast surface antigen of approximately 110 kDa.
Conclusions:
- A novel monoclonal antibody successfully identified a specific lymphokine receptor on chicken lymphoblasts.
- The identified 110 kDa receptor may be functionally and structurally analogous to the interleukin 2 receptor gamma subunit in mammals.
- This research provides a tool for further studying chicken immune cell activation and lymphokine signaling.
