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Related Experiment Videos

Mammalian small molecule methyltransferases: their structural and functional features.

M Fujioka1

  • 1Department of Biochemistry, Toyama Medical and Pharmaceutical University, Faculty of Medicine, Japan.

The International Journal of Biochemistry
|December 1, 1992
PubMed
Summary

Mammalian small molecule methyltransferases share common structural and functional traits, including similar molecular weights and conserved sequence motifs. These enzymes utilize S-adenosylmethionine and often feature vicinal thiols crucial for their catalytic activity.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Molecular Biology

Background:

  • S-adenosylmethionine-dependent methyltransferases are vital enzymes in mammals.
  • These enzymes play critical roles in various biological processes through methylation.
  • Understanding their structure and function is key to comprehending cellular metabolism.

Purpose of the Study:

  • To review the structural and functional characteristics of mammalian small molecule methyltransferases.
  • To identify conserved features across different enzymes within this class.
  • To highlight key aspects of their catalytic mechanisms and substrate binding.

Main Methods:

  • Literature review of existing studies on mammalian methyltransferases.
  • Analysis of sequence data to identify common motifs.

Related Experiment Videos

  • Examination of kinetic and structural data to understand enzyme function.

    • Main Results:

    • Mammalian small molecule methyltransferases exhibit conserved protomer molecular weights (25,000-35,000).
    • Two common sequence motifs are present in all known enzymes of this class.
    • These enzymes bind S-adenosylmethionine in their free form.
    • Most mammalian methyltransferases possess vicinal thiols in their active sites.

    Conclusions:

    • Mammalian small molecule methyltransferases are a conserved enzyme family with shared structural and functional properties.
    • The presence of specific sequence motifs and vicinal thiols suggests a common evolutionary origin and catalytic strategy.
    • Further research into these conserved features can elucidate their roles in health and disease.