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TOR complex 1 includes a novel component, Tco89p (YPL180w), and cooperates with Ssd1p to maintain cellular integrity

Aaron Reinke1, Scott Anderson, J Michael McCaffery

  • 1Section of Molecular and Cellular Biology and Center for Genetics and Development, Division of Biological Sciences, University of California, Davis, California 95616, USA.

The Journal of Biological Chemistry
|January 23, 2004
PubMed

Insights

Researchers identified new components of yeast TORC1 and TORC2 complexes, revealing a link between TORC1 and cellular integrity maintenance, crucial for rapamycin

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cellular Signaling

Background:

  • The target of rapamycin (TOR) pathway regulates cell growth and metabolism.
  • TOR kinases, Tor1p and Tor2p, form distinct complexes: TORC1 and TORC2.
  • TORC1 is sensitive to rapamycin and involves Lst8p and Kog1p.

Purpose of the Study:

  • To comprehensively characterize protein-protein interactions within yeast TORC1 and TORC2.
  • To identify novel components of TORC1 and TORC2.
  • To elucidate the role of TORC1 in cellular integrity.

Main Methods:

  • Co-purification assays to identify interacting proteins.
  • Genetic analysis of yeast deletion mutants.
  • Phenotypic analysis including rapamycin sensitivity and cellular integrity assays.

Main Results:

  • Identified Tco89p as a novel component of TORC1 and Bit61p in TORC2.
  • Deletion of TOR1 or TCO89 caused rapamycin hypersensitivity and decreased cellular integrity.
  • Linked Ssd1p to Tap42p, establishing a connection between TORC1 and cellular integrity.

Conclusions:

  • TORC1 and TORC2 composition is further defined by novel components Tco89p and Bit61p.
  • TORC1 plays a role in maintaining cellular integrity, potentially mediated by Ssd1p.
  • A novel link between the TOR pathway and cellular integrity maintenance is established.

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