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Published on: October 28, 2014
Automated flow cytometry analysis of peritoneal dialysis fluid
Joris Penders1, Tom Fiers, Annemieke M Dhondt
1Department of Clinical Chemistry, Microbiology and Immunology, Ghent University Hospital, Ghent, Belgium.
Summary
The Sysmex UF-100 flow cytometer provides a rapid and accurate leukocyte count for peritoneal dialysis fluid. Its bacterial channel shows promise in detecting infections, outperforming Gram stains for positive cultures.
Area of Science:
- Clinical Chemistry
- Medical Technology
- Flow Cytometry
Background:
- The Sysmex UF-100 flow cytometer automates urinalysis.
- Its utility in analyzing peritoneal dialysis (PD) fluid was unexplored.
Purpose of the Study:
- Evaluate the Sysmex UF-100 for PD fluid analysis.
- Compare flow cytometry data with traditional methods.
Main Methods:
- Correlated UF-100 data with microscopy and biochemical data in 135 PD samples.
- Performed microbiological analysis in 63 suspected peritonitis cases.
Main Results:
- Good agreement between UF-100 and microscopy for leukocytes (r=0.825).
- UF-100 bacterial counts showed unreliability due to platelet interference and background noise.
- Yeast cells were detected in spiked samples.
Conclusions:
- Flow cytometry with UF-100 offers rapid, reliable leukocyte counts in PD fluid.
- The bacterial channel's sensitivity for positive cultures exceeds Gram stain.
- Provides semi-quantitative data on yeast presence.
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