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Related Experiment Videos

Multifiber, multiwavelength, fiber optic fluorescence spectrophotometer.

D Lipson1, N G Loebel, K D McLeaster

  • 1Eli Lilly and Company, Indianapolis, IN 46285.

IEEE Transactions on Bio-Medical Engineering
|September 1, 1992
PubMed
Summary

A novel fiberoptic spectrofluorimeter enables automated, remote control of multiple biosensors. This advanced instrument achieves high sensitivity and low detection limits for precise chemical analysis.

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Area of Science:

  • Analytical Chemistry
  • Biomedical Engineering
  • Optical Instrumentation

Background:

  • Remote sensing and multiplexed analysis are crucial for modern biosensing applications.
  • Existing spectrofluorimeter systems may lack the automation and sensitivity required for complex environments.

Purpose of the Study:

  • To develop and characterize a novel fiberoptic spectrofluorimeter for automated, multiplexed biosensing.
  • To evaluate the performance of the instrument in terms of sensitivity, sampling volume, and operational flexibility.

Main Methods:

  • Construction of a fiberoptic spectrofluorimeter with remote, automated control for up to 18 biosensors.
  • Independent adjustment of excitation/emission wavelengths, optical bandwidths, signal gain, phase, integration time, and duty cycle.

Related Experiment Videos

  • Implementation of synchronous demodulation for enhanced signal-to-noise ratio and ambient light operation.

    • Main Results:

    • Achieved a detection limit below 1 ng/mL for aqueous sodium-fluorescein with a signal-to-noise ratio greater than unity.
    • Demonstrated effective sampling volumes as low as 0.02 microL.
    • Observed characteristic fiberoptic sampling behavior with asymptotic signal magnitudes.

    Conclusions:

    • The developed fiberoptic spectrofluorimeter offers a robust platform for sensitive, automated, and multiplexed biosensing.
    • The instrument's design facilitates operation in diverse conditions, including ambient lighting.
    • Potential applications include environmental monitoring, medical diagnostics, and high-throughput screening.