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Related Experiment Videos

Contrasting roles for CXCR2 during experimental colitis.

Maureen N Ajuebor1, John Zagorski, Steven L Kunkel

  • 1Gastrointestinal Research Group, Faculty of Medicine, University of Calgary, Calgary, AB, Canada T2N 4N1. ajuebor@ucalgary.ca

Experimental and Molecular Pathology
|January 24, 2004
PubMed
Summary

Chemokine receptor CXCR2 drives early neutrophil recruitment in TNBS-induced colitis. However, CXCR2 is not essential for neutrophil accumulation in the later stages of this inflammatory bowel disease model.

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Area of Science:

  • Gastroenterology
  • Immunology
  • Inflammation Research

Background:

  • Neutrophil recruitment to the colon is critical in inflammatory bowel diseases (IBDs).
  • The chemokine receptor CXCR2 (C-X-C chemokine receptor type 2) mediates neutrophil recruitment in various inflammatory conditions.

Purpose of the Study:

  • To investigate the role of CXCR2 in neutrophil accumulation during TNBS-induced colitis in rats.
  • To assess the impact of CXCR2 neutralization on early and late-phase neutrophil infiltration.

Main Methods:

  • Induction of colitis using trinitrobenzene sulfonic acid (TNBS) in rats.
  • Administration of a CXCR2 neutralizing antibody at different time points.
  • Quantification of colonic neutrophil infiltration at 8 hours and 7 days post-TNBS.

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Main Results:

  • CXCR2 expression and neutrophil infiltration were significantly increased in colitic rats.
  • A single dose of CXCR2 antibody reduced neutrophil accumulation during the early phase (8 hours).
  • Chronic CXCR2 antibody administration did not affect neutrophil accumulation in the late phase (7 days).

Conclusions:

  • CXCR2 plays a key role in initiating neutrophil recruitment during the early phase of acute TNBS-induced colitis.
  • Early colonic neutrophil accumulation is CXCR2-dependent, while late-phase accumulation is CXCR2-independent.