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Determination of celecoxib in human plasma using solid-phase extraction and high-performance liquid chromatography
H-H Sherry Chow1, Nathan Anavy, Dawn Salazar
1Arizona Cancer Center, The University of Arizona, 1515 N Campbell Avenue, Tucson, AZ 85724-5024, USA. schow@zcc.arizona.edu
Journal of Pharmaceutical and Biomedical Analysis
|January 24, 2004
Summary
A new high-performance liquid chromatography (HPLC) method accurately measures celecoxib in human plasma. This validated assay is crucial for clinical studies involving celecoxib.
Area of Science:
- Analytical Chemistry
- Pharmacokinetics
Background:
- Celecoxib is a widely used nonsteroidal anti-inflammatory drug.
- Accurate quantification of celecoxib in biological matrices is essential for pharmacokinetic studies and therapeutic drug monitoring.
Purpose of the Study:
- To develop and validate a simple, sensitive, and reliable reversed-phase high-performance liquid chromatography (HPLC) method for determining celecoxib concentrations in human plasma.
Main Methods:
- Solid-phase extraction (SPE) using C18 cartridges for sample preparation.
- Chromatographic separation on a Nova Pak C8 column.
- Detection using an ultraviolet (UV) detector at 215 nm.
- Quantification using an internal standard (SC-236).
Main Results:
- The method demonstrated excellent recovery (>88%) and resolution of celecoxib from plasma components.
- Linear calibration curves were obtained over a wide concentration range (40-4000 ng/ml).
- The assay exhibited good precision, with inter- and intra-day coefficients of variation below 12% and 5%, respectively.
Conclusions:
- The developed HPLC method is suitable for the accurate and precise determination of celecoxib in human plasma.
- This validated assay can be effectively applied to analyze plasma celecoxib levels in clinical trials, such as the reported Phase II study.