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Related Experiment Videos

Assessment of complement activation in vivo.

M Oppermann1, U Höpken, O Götze

  • 1Department of Immunology, University of Göttingen, Germany.

Immunopharmacology
|September 1, 1992
PubMed
Summary

New assays measuring complement fragments directly in plasma offer superior assessment of complement activation compared to traditional methods. Accurate in vivo complement activation analysis requires measuring both native proteins and their breakdown products.

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Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Traditional hemolytic assays and immunochemical quantitation of native complement proteins are limited in assessing in vivo complement activation.
  • The complement system's complexity, with independent protein metabolism, complicates accurate analysis.
  • Rapid in vivo metabolism of complement proteins adds further challenges to traditional assessment methods.

Purpose of the Study:

  • To introduce and evaluate novel assays for directly determining complement fragments in plasma.
  • To highlight the advantages of neoepitope-specific monoclonal antibody assays over native protein quantitation.
  • To emphasize the need for a comprehensive approach to accurately estimate in vivo complement activation.

Main Methods:

  • Utilizing monoclonal antibodies specific for activation-dependent neoepitopes.

Related Experiment Videos

  • Directly determining complement fragments in plasma.
  • Simultaneous determination of native complement components and activation products.

    • Main Results:

    • Assays based on activation-dependent neoepitopes allow direct determination of complement fragments in plasma.
    • These neoepitope-specific assays are superior to quantitating native complement proteins.
    • Elevated plasma fragment levels require consideration of factors like receptor binding, protease degradation, and clearance.

    Conclusions:

    • Direct measurement of complement fragments using neoepitope-specific assays provides a more accurate assessment of in vivo complement activation.
    • Accurate in vivo complement activation estimation necessitates the simultaneous measurement of both native complement components and their activation products.
    • Understanding catabolic and clearance factors is crucial when interpreting elevated complement fragment levels.