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Complexity of dsRNA mycovirus isolated from Fusarium graminearum

Yeon-Mee Chu1, Won-Seok Lim, Sang-Jin Yea

  • 1School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, Korea.

Virus Genes
|January 24, 2004
PubMed

Insights

Researchers screened Fusarium graminearum isolates for mycoviruses, finding genomic diversity in double-stranded RNA (dsRNA) mycoviruses. Virus impact on fungal traits depends on host-virus interactions, not just dsRNA presence.

Area of Science:

  • Mycology
  • Plant Pathology
  • Virology

Background:

  • Fusarium graminearum causes significant scab disease in Korean small grains.
  • Previous studies linked some dsRNA mycoviruses to altered fungal morphology and virulence.

Purpose of the Study:

  • To screen F. graminearum isolates for dsRNA mycoviruses.
  • To investigate the genomic diversity and transmission of these mycoviruses.
  • To determine the impact of mycovirus infection on fungal traits.

Main Methods:

  • Screening of 827 F. graminearum isolates from barley and maize for dsRNA.
  • Characterization of dsRNA mycovirus segments and their transmission through spores.
  • Analysis of host-virus interactions affecting fungal morphology and virulence.

Main Results:

  • 19 out of 827 isolates contained dsRNAs; 10 new isolates were identified with dsRNA mycoviruses.
  • Mycoviruses comprised 2-4 dsRNA segments (1.7-10 kbp).
  • dsRNAs were transmissible (30-100%) via conidia and ascospores, with no consistent effect on colony morphology.

Conclusions:

  • Genomic diversity exists among dsRNA mycoviruses infecting F. graminearum.
  • The impact of dsRNA mycovirus infection on fungal morphology and virulence is specific to the host-virus interaction.
  • Mere presence of dsRNAs does not solely determine phenotypic changes in the fungal host.

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